PHCbi
PHC株式会社 バイオメディカ機器 Live Cell Metabolic Analyzer (LiCellMo) Application Note

Application Note

Application Note List

In-Line monitoring of culture medium using Live cell metabolic analyzer

A case study demonstrating the real-time measurement of changes in glucose and lactate concentrations during Jurkat cell culture. It shows that changes in glucose and lactate can be assessed with an accuracy comparable to conventional methods that involve measuring the culture medium supernatant at each time point.

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Visualizing the metabolic profile of immune cells with metabolic inhibitors using the Live cell metabolic analyzer

A case study investigating how glucose and lactate concentrations change during culture when administering drugs that inhibit different metabolic activities in two leukemia-derived cell lines. The observed changes in metabolic rates suggest an accompanying shift in the overall cellular state.

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Metabolic rate monitoring of undifferentiated iPS cells and expression analysis of differentiation markers

A case study observing changes in glucose and lactate concentrations while culturing iPS cells using an undifferentiated maintenance protocol with regular medium exchanges. Over a 7-day culture period, it shows that cell numbers increase while the metabolic rate decreases in the later stages of culture. This indicates potential applications in developing protocols to improve the quality of undifferentiated iPS cells.

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Metabolic shift monitoring of immune cells during differentiation

A case study recording real-time changes in glucose and lactate concentrations upon administering a drug that promotes differentiation into macrophages in a monocytic leukemia-derived cell line. It chronologically demonstrates that glucose consumption and lactate production rates change during the differentiation from monocytes to macrophages.

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Investigation of a novel cardiotoxicity evaluation method using iPS-derived cardiomyocytes

A case study demonstrating the highly sensitive detection of metabolic changes induced by cardiotoxic substances by observing the metabolic rates of glucose and lactate in human iPS cell-derived cardiomyocytes. The results suggest that cardiotoxicity can be detected at lower concentrations compared to conventional methods.

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